Metabolomics

• Focus of the subject is to introduce novel analytical methodologies with application in pre-clinical, clinical trials, cell models and plant experiments.
• The objective of the course is to give students new knowledge and widening of the knowledge acquired in other courses using novel holistic approaches for metabolite evaluation in plants, animals, microbial systems and their interactions.

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• The course will be composed of following chapters:
• Metabolome description
• Preparation of experimental design with focus -on decreasing of system variability.
• Sample preparation of food and plant material
• Analytical methods in metabolomics
• Data processing using univariate and multivariate statistical analysis
• Annotation and confirmation of metabolites
• Structural elucidation of new compounds
• Inclusion of metabolites into biosynthetic pathways.
• Using stable isotopes for pathway determination
• Examples of metabolomic studies on plant models, on modelst in viticulture and enology
• Integration of metabolomics with other »omics« approaches as genomics, transcriptomics and proteomics.

• Knowledge and understanding of plant metabolic processes and tools for their evaluation.
• Applications.
• Presentation of ongoing research.
• Reflexion.
• The ability of understanding novel analytical methodologies including their application in pharmaceutical, nutritional and biomedicine practice.
• Transmissible skills.
• Critical review of up-to-date research literature, currently developing metabolome databases and data processing on-line platforms.

• The HANDBOOK of metabonomics and metabolomics edited by John C. Lindon, Jeremy K. Nicholson and Elaine Holmes. - 1st ed. Amsterdam ; Oxford : Elsevier, 2007. - 1 online resource (x, 561 p.) : ill. (some col.), ISBN 978-0-444-52841-4 Catalogue
• GENOMICS, proteomics, and metabolomics in nutraceuticals and functional foods / editors, Debasis Bagchi, Francis C. Lau, Manashi Bagchi. - Ames (Iowa) : Wiley-Blackwell, cop. 2010. - XIV, 338 str., 16 str. ISBN 978-0-8138-1402-5 E-version
• METABOLOMICS in practice : successful strategies to generate and analyze metabolic data / edited by Michael Lämmerhofer and Wolfram Weckwerth. - Weinheim : Wiley-VCH, cop. 2013. - XXV, 415 str. ISBN 978-3-527-65589-2 Catalogue
• NUTRITIONAL genomics : discovering the path to personalized nutrition / edited by Jim Kaput and Raymond L. Rodriguez. - Hoboken (New Jersey) : Wiley-Interscience, cop. 2006. - XXV, 469 str. ISBN 0-471-68319-1 E-version
• PLANT metabolomics : methods and protocols / edited by Nigel W. Hardy, Robert D. Hall. - New York : Humana Press : Springer, cop. 2012. - XIII, 340 str. ISBN 978-1-61779-593-0 Catalogue
• Slides
• Moodle classrom with e-materials

Discussion with other students and the lecturer responsible for the course (50%), Oral exam (50%)

Associate professor for the field of Food at UNG and dean of the School for Viticulture and Enology.

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