In vitro isolation of nanobodies

In vitro isolation of nanobodies for selective Alexandrium minutum recognition: A model for convenient development of dedicated immuno-reagents to study and diagnostic toxic unicellular algae

Importance:

Immunoregents for toxic microalgae research and diagnostics are rare. UNG researchers together with the Oceanographic Institute of Triest demonstrated that it is possible to recover very specific nanobodies by direct selection of a pre-immune library in the presence of whole microalgae cells. The expression of such nanobodies as fusion with fluorescent proteins enabled the direct visualization of the target cells

REFERENCE

Mazzega E, Beran A, Cabrini M, de Marco A (2019) In vitro isolation of nanobodies for selective Alexandrium minutum recognition: A model for convenient development of dedicated immuno-reagents to study and diagnostic toxic unicellular algae. Harmful Algae 82:44-51